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- Contents
- Table of Contents
- Bookmarks
Table of Contents
- Welcome
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Table of Contents
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- Copyright and Trademarks
- Symbols of Qsep Series
- Applications
- Packing List
- Cautions
- 1 System Overview
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Qsep ™ Instrument
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Qsep ™ Front View
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Qsep ™ Back View
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Qsep ™ Top View
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Qsep ™ Instrument
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Qsep ™ Front View
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Qsep ™ Back View
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Qsep ™ Top View
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Hardware Requirements
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- 2 Installation Instruction
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Environment Requirements
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Power Considerations
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Hardware Installation
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Qsep ™ Installation
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Qsep ™ Installation
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- 3 Quick Start
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Qsep ™ Quick Start
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Qsep ™ Quick Start
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Qsep
Qsep
&
Operation Manual
1
100
--- Hardware
Revolution
BiOptic Inc.,
4F., No.108-3, Minquan Rd., Xindian Dist., New Taipei City 23141, Taiwan (R.O.C.)
www.bioptic.com.tw
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Summary of Contents for BiOptic Qsep1
- Page 1 Qsep Qsep & Operation Manual --- Hardware Revolution BiOptic Inc., 4F., No.108-3, Minquan Rd., Xindian Dist., New Taipei City 23141, Taiwan (R.O.C.) www.bioptic.com.tw...
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Page 2: Welcome
Copyright and Trademarks Copyright © BiOptic Inc. All rights reserved. Reproduction, adaptation, or translation of this manual is prohibited without prior written permission of BiOptic Inc., except as allowed under the copyright laws. ™ Series and ™ are registered trademarks of BiOptic Inc. -
Page 3: Table Of Contents
Welcome ........................2 Copyright and Trademarks ..................2 Symbols of Qsep Series ....................2 Applications ........................4 Packing List ........................4 Cautions ........................5 1. System Overview ...................... 7 ™ Instrument .................... 8 1.1 Qsep ™ Front View ..................8 1.1.1 Qsep ™... -
Page 4: Applications
BEFORE ATTEMPTING TO OPERATE THE INSTRUMENT, READ ALL PRODUCT MANUALS AND FOLLOW THE INSTRUCTIONS. BiOptic Inc. assumes no liability whatsoever for any personal injury, property damage, or other loss resulting from not complying or familiar with the manuals, or improper operation of the devices. -
Page 5: Cautions
™ Series away from other electronic device and voltage sources. Qsep Keep Only the components and consumables provided by BiOptic Inc. are suggested to use. DO NOT perform the following actions: •... - Page 6 ™ Series are high-voltage Single-channel electrophoresis Qsep Warning: system. Please follow the operation manual and laboratory safety guidelines for system operation. Do not remove covers. For operation and safety questions, please contact BiOptic Inc. at the official website or with your local BiOptic representatives.
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Page 7: System Overview
1. System Overview ™ Series are a fully-automated CE system developed by BiOptic Inc., which uses Qsep pen-shaped disposable gel-cartridges to improve efficiency. Time-consuming manual procedures such as gel preparation, sample loading, and capillary changing are no longer required. Further, the information will be obtained easily with the fully- ™... -
Page 8: Qsep ™ Instrument
Qsep ™ Instrument ™ Front View Qsep 1.1.1 Figure 1-1 ™ front view Qsep A. High voltage alert lamp: The red LED blinks when the high voltage source of the capillary electrophoresis is ON. B. Main Power switch: The blue LED lights up when it is turned on. C. - Page 9 Figure 1-2 Rotation platform...
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Page 10: Qsep ™ Back View
™ Back View Qsep 1.1.2 Figure 1-3 Qsep ™ back view A. Air pressure gauge: ™. Qsep Display the value of the pressure operating in B. Air flow Inlet Port: Connect to an external pump using an 1/8 inches tube. C. -
Page 11: Qsep ™ Top View
™ Top View Qsep 1.1.3 Figure 1-4 Qsep ™ top view A. Cartridge door... -
Page 12: Qsep ™ Instrument
™ Instrument Qsep Qsep ™ Front View 1.2.1 A. Indicator lamp: B. SD Card Port C. Cartridge Door D. Sample Door and Sample Tray... -
Page 13: Qsep ™ Back View
™ Back View Qsep 1.2.2 A. Power Connector B. Main Power switch C. Air flow Inlet Port: Connect to an external pump using an 1/8 inch tube. D. Cable Port... -
Page 14: Qsep ™ Top View
™ Top View Qsep 1.2.3 A. Cartridge door... -
Page 15: Hardware Requirements
1.3 Hardware Requirements Minimum Recommended Intel Core i3 530 Intel Core i7 2933MHz (Dual Core) 2.8 GHz 4G RAM or More 8G RAM or More 320GB or More 500GB or More PORT USB 2.0 x 1 USB 3.0 x 1 INTERNET Required... -
Page 16: Installation Instruction
2. Installation Instruction A suitable operating environment is essential to ensuring the best performance of ™ Series. Qsep 2.1 Environment Requirements ⚫ ™ Series are 18°C~26°C (65°F ~78°F). Qsep The optimal operating temperature of ⚫ Qsep The optimal operating humidity of ™... -
Page 17: Power Considerations
2.2 Power Considerations Qsep ™ instrument is 24V DC, and the maximum current is The input voltage of 1.25A. A suitable power adapter with cord, SINPRO MPU32B-108, is included in the package (100~240V AC, 47~63Hz). The dimensions are 38cm(L)*30cm(W)*40cm(H) and weight of the instrument is 15Kg. Direct control the instrument with USB cable. Connect the air source within the range of 50psi~75psi. -
Page 18: Hardware Installation
2.3 Hardware Installation ™ Series instrument on a stable platform, and ensure it is away from Qsep Place ™ Series need to be Qsep water and any other high-power electronic devices. operated in a clean and well-ventilated environment. Remove the power adapter from the package and attach it with a suitable power cord. -
Page 19: Qsep ™ Installation
Qsep ™ Installation 2.3.2 Prepare a computer with Windows operation system (i.e. Windows 10). ™, user could use the cable or WiFi function to connect the instrument Qsep ™. Qsep with your computer after Turn on Turn on the power, wait 25 second for WiFi function initialization (Green light ™. -
Page 20: Quick Start
3. Quick Start 3.1 Qsep ™ Quick Start Ensure the SD card is properly plugged in the socket (Fig. 1 and 2). SD card needs to be plugged-in before power on. The SD card CAN NOT be initialized with hot plugging. - Page 21 The password will show in the sticker which is behind the instrument. It is the same as the middle 8 digits of instrument serial number (same as SSID name) by blinding the prefixal “Qsep1” (Fig. 7). Qsep *If your computer can’t find the SSID of...
- Page 22 - Please check the wi-fi IP assignment is “Automatic (DHCP)” - Allow Q-Analyzer pass through firewall or turn off the firewall Check the LED status. Double click the Q-Analyzer. Q-Analyzer user interface: 7-1. Select “Network” mode and click “Search” *After “Search” the instrument ID will display *Please confirm the instrument ID is the connected instrument Qsep 7-2.
- Page 23 Prepare buffer and alignment marker in 0.2ml PCR tubes and allocate at the corresponding position on the sample tray *For 12-well sample tray, user needs to use 0.1ml tube (C104252) for sample and size marker *S/P/C well can only place 0.2ml PCR tubes *Make sure no air bubble appears in each tube Click “Change Sample”...
- Page 24 13. Close the cartridge door and click “Latch”. *The cartridge information will be displayed after latched. 14. Cartridge Calibration: New cartridge needs to be calibrated before using. Please proceed with the following steps. 14-1. Click “HV Check”. * The storage and transportation condition may influence the Gel-matrix and cause unstable current.
- Page 25 During the “HV Check”, the last LED will show red light Blue→ SD card green→ WiFi connectable red→ HV check 14-2. Click “Calibrate”. *The “Calibrate” button will only display after HV check passed. * For troubleshooting, please refer to cartridge unpacking guide for details. 15.
- Page 26 15-2. Click on “Method” to select a proper method for your application. 15-3. Click on “Result Name ” and enter the name of result files. 15-4. Click the icon “Para” and set the parameters. (Baseline Factor, Peak Threshold, Calculate…etc.). 16. Click “Run” to start the process.
- Page 27 During Calibration Please check the Alignment Marker has been located at correct position. Software will recognize two peaks from Alignment Marker signal. DO NOT use Size Marker or DNA sample doing “Calibrate”. Brief introduction of the Signal Chart:...
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Page 28: Qsep ™ Quick Start
™ Quick Start 3.2 Qsep 1. Turn on the power. Q-Analyzer. 2. Double click the 3. Main window appears. 4. Click “Connect” to link the instrument. 5. Cartridge and Buffer preparation:... - Page 29 5-1. Unpacking Cartridge: Please follow the steps of “Unpacking guide” inside the cartridge box. 5-2. Add 1X Separation Buffer into “S” well and add diH O into others. * Buffer height should be equal to the groove of wells. 5-3. Alignment Marker preparation: Add ≥...
- Page 30 9. Click “Change Sample” and place your samples. (≥ 20 μl) * Spin down and make sure the sample is in the bottom of tube and no air bubble appears. 10. According to cartridge unpacking guide, open the cartridge door and insert cartridge.
- Page 31 Cartridge information will be presented on the main window after cartridge latched. 12-1. New Cartridge Calibration: New cartridge needs to be calibrated before using. Please proceed with the following steps. • Click “Confirm”. • Click “HV Check”. * The storage and transportation condition may influence the gel-matrix and cause unstable current.
- Page 32 13-1. Click the “Sample Position”, mark the position of sample on the plate and then press “OK”. 13-2. Click “Method” to select analysis method. * If you want to analyze the samples with Alignment Marker, please choose the appropriate Alignment Marker and then place it on the corresponding √...
- Page 33 13-4. Click the icon “Para” and you can set the parameters. (Baseline Factor, Peak Threshold, Calculate…etc.) 14. Click “Run” to start the process. During Calibration Please check the Alignment Marker has been located in correct position. Software will recognize two peaks from Alignment Marker signal. DO NOT use Size Marker or DNA sample doing “Calibrate”...
- Page 34 Introduction of the Control Panel...
This manual is also suitable for:
Qsep100
