Bioptic Qsep100 Manuals (2024)

Welcome

2

Copyright and Trademarks

2

Symbols of Qsep Series

2

Table of Contents

3

Applications

7

Packing List

7

Cautions

8

System Overview

10

• Pre-Program Method and Cartridge

  11

  • Pre-Program Method

    11

• System Installation

  12

  • Software Requirements

    12

Q-Analyzer Installation Instruction

13

• Software Installation

  13

• Software Mode

  18

  • USB Software Key

    20

  • Q-Editor License

    23

Start to Use Qsep™ Series

26

• Operation of Qsep™ Series

  27

  • Connection Assistance

    28

  • Sequence and Method Setting

    32

• Cartridge Calibration

  40

• Edit Sample Position Settings

  42

• Recalibration

  44

• Capillary Clog Check

  45

• Purge Check Function

  48

• Home Function

  50

Q-Analyzer™ User Interface

51

• Main Window

  51

  • System Overview

    52

  • System Status Column

    53

• Function Bar and Toolbar

  54

  • Instrument

    54

  • Results

    59

  • Comparison

    65

    See Also

    BIOPTIC QSEP1 OPERATION MANUAL Pdf DownloadQsep Bio-Fragment Analyzer

  • Real Time

    66

• Menu

  67

  • File

    67

  • Edit

    70

  • Tool

    74

  • View

    77

  • Window

    78

  • Setting

    78

  • Language

    79

  • Help

    80

• Control Panel

  82

  • Main

    82

  • Method

    84

  • Direct Control (Partial Advanced Only)

    86

  • Board Setting (Advanced Only)

    87

Result/Data Analysis

88

• Result for Data Display

  88

  • Calculation

    91

  • Reference Marker Table

    93

  • Improve Calculate Accuracy

    95

• Comparison

  96

  • Export the Report of Compare Result

    103

• Total RNA Quality Analysis

  105

• Smear DNA Analysis (Gdna QC for NGS)

  107

• Peak Calling Function for Clinical Testing

  109

Appendix A-Troubleshooting

112

Unpacking, Packing Process and Installation

112

• Instrument

  112

• Q2：What Are the Minimum Computer Requirements and Recommendations

  112

• Q3：What Is the Size of the Result File

  113

• Q4：Do We Need to Install the Software First or Connect the Cable First

  113

• Q5：Can Power Adaptors be Used Alternately

  113

Instrument Not Working or Working Abnormally

113

Before Use

113

• Q1：Pressing the Machine Power, but Have no Response

  113

• Q2：How Should We Do When PC Cannot Detect the Connection of Qsep

  114

• Q3：How Should We Do When PC Cannot Connect with Qsep Wifi Mode

  114

• Q4：What Does the Light of Qair Mean

  115

During Use

115

• Q5：If the Buffer Tray Can't Move Smoothly, How Should We Do

  115

• Q6：The Qair Works Frequently, over 3 Times/Min

  117

• Q7：Jet Error Window Pop-Out

  117

Cartridge Issues

118

Before Use

118

• Q1：HV Check Failed or Calibration Failed

  118

• Q2：What's Wrong with the Pop-Out Error Window

  119

During Use

119

• Q3：How to Check if the Cartridge Is Clogged or Not and How to Rescue the Dry out Cartridge

  119

• Q4：What if the Cartridge Has Expired, but Still Has Runs Left

  120

• Q5：What if the Cartridge Has Sucked the Mineral Oil? How to Recover It

  120

• Q6：Anything Needs to Notice During Insert or Take out the Cartridge

  121

Storage

122

• Q7：How to Store the Cartridge? and Relative Buffer

  122

Questions of Operation

123

• Q1：How Do I Place the Qsep

  123

  • Alignment Marker and Size Marker

    123

• Q2：How Do I Prepare the Unknown Sample before Qsep Analysis

  123

• Q3：The Minimum Volume of Sample

  123

• Q4：The Sample Consumption (Per Run)

  124

• Q5：What Is the Air Pressure Range of each Instrument

  124

• Q6：What if We Use the Wrong Buffer

  124

• Q7：Will Dye or Organic Solvent Influence Cartridge? How to Pretreat the Sample

  125

• Q8：What Is the Difference between Basic Key and Advance Software Key

  125

• Q9：What Is the Difference between Q-Analyzer TM , Q-Editor TM and Q-Viewer TM

  126

• Q10：Why Should I Do Recalibration

  127

Unexpected Results

128

• Q1：What Kind of Conditions will Cause Unstable Current

  128

• Q2：What to Do if Alignment Marker and Samples Are Migrating too Slowly and the Upper Alignment Marker Does Not Appear on the Result

  128

• Q3：Identify Wrong Alignment Marker or Size Marker

  129

• Q4：The Calculated Size Is Wrong

  131

• Q5：Is the Concentration Display in Result Table Reliable

  131

• Q6：The Current Is Stable, but There Is no Signal at All, Even the Alignment Marker Does Not Appear. RFU Near Zero

  131

• Q7：The Current Is Stable, but There Is no Signal Except the Alignment Marker

  132

• Q8：One of the 4 Channel Shows Different Result with Others

  132

Specific Application Issues (RNA, Protein

133

• R1 RNA Cartridge

  133

• Q1：When Running RNA Samples, Are There any Special Things to Consider

  133

• Q2：What Is the Criteria of RQN

  133

• Q3：Why the Result Shows "N/A" in RQN

  134

• P2 Protein Cartridge

  135

• Q4：What Protein Alignment Marker Should I Use

  135

• Q5：Which Instrument Can I Use to Analyze Protein Sample? or What Labeling Dye Should I Choose

  136

• Q6：How to Do the Labeling Process

  136

Maintenance

136

• Q1：How Often Should I Change the Marker and Buffer

  136

• Q2：What Happens if I Don't Change the Filter for a Long Time

  136

• Q3：How to Clean the Instrument Regularly

  136

Others

137

• Q1：Should We Turn off the Instrument and Pump Every Day

  137

• Q2：How Should We Do before Discard the Cartridge

  137

• Report to Bioptic

  138

2

• Table of Contents

  3

2

2

4

4

5

7

• Qsep ™ Instrument

  8

  • Qsep ™ Front View

    8

  • Qsep ™ Back View

    10

  • Qsep ™ Top View

    11

• Qsep ™ Instrument

  12

  • Qsep ™ Front View

    12

  • Qsep ™ Back View

    13

  • Qsep ™ Top View

    14

• Hardware Requirements

  15

16

• Environment Requirements

  16

• Power Considerations

  17

• Hardware Installation

  18

  • Qsep ™ Installation

    18

  • Qsep ™ Installation

    19

20

• Qsep ™ Quick Start

  20

• Qsep ™ Quick Start

  28