Jack Frost Mushroom Liquid Culture (2024)

FAQs

How long does mycelium take to grow in liquid culture? ›

Within 7 – 14 days you'll see the mycelium has 'clouded' the culture and expanded throughout; this is aided with semi-regular mixing/shaking. This can be achieved by use of a magnetic stirrer, a bolt in the bottom or just swirling by hand. Things you'll need: AirPort Jar – Here is my guide on how to make them.

While liquid cultures can be stored for many months, we recommend using them within six months for the best results. In our experience, liquid cultures of nearly any variety can grow even after a year of storage in the refrigerator.

Making Liquid Culture Solution:

Our favorite recipe for making liquid culture is a sugary solution not greater than 4% by Weight, we usually use unbleached corn syrup or honey for our sugar source. Therefore the ratio we would want is 4 grams of sugar to 100 ml of water.

A good liquid culture will often be a tiny bit cloudy and then clear up.

Mycelium transferred from agar plates into liquid culture will grow until the container is fully colonised and ready to incoulate your spawn. You can also inoculate freshly made jars of liquid culture with a sample of your now colonised culture to generate even more…

Liquid Culture vs.

spores revolves around speed and diversity. Liquid culture accelerates mycelium growth, boasts higher success rates, and lowers contamination risks—ideal for beginners and seasoned growers. Spores, however, embrace genetic diversity, paving the way for a more organic cultivation process.

All you need to do is inject around 1ml of spores into the liquid culture vial, then shake. There's no need for a sterile environment, and you'll cultivate a high-quality liquid culture in return.

There are several opinions on this, but generally growers recommend swirling, stirring or shaking your jar once a day. If you have a very slow-growing strain, you may only need to shake it every second day when you see a clump or cloud of mycelium forming instead of small strands.

If there is any mycelium, it looks weak and poor and not white, aggressive and fluffy. Contaminated LC jars will be cloudy and smell sour. There will be a sediment at the bottom instead of the beautiful cloud formations of healthy mycelium.

Inject approximately 1-2 ml of liquid culture into a quart-sized jar of grain. Use 5-10 ml of liquid culture to inoculate a 5 lb bag of sterilized grain. A jar or bag of grain should be fully colonized in 14-45 days, depending on the mushroom species and environmental conditions.

How much liquid culture is in a grain jar? ›

Usually about 1 – 2 CC's of liquid culture is sufficient per quart sized grain jar. You can also inoculate the grain jar without even opening the lid, by simply injecting the liquid culture right though the filter on the top of the jar.

The inoculation process is also far shorter in liquid cultures. You can expect mushrooms to only incubate for 10 days to three weeks, which is significantly less than normal. The level of mycelium growth is practically limitless, giving you a high yield every time.